Nanocrystalline hydroxyapatite and zinc-doped hydroxyapatite as carrier material for controlled delivery of ciprofloxacin

In bone disorders infections are common. The concentration of majority of antibiotics is very low in the bone tissue. A high local dose can be obtained from the ciprofloxacin-loaded hydroxyapatite nanoparticles. The present study is aimed at developing the use of hydroxyapatite and zinc-doped hydroxyapatite nanoparticles as a carrier for ciprofloxacin drug delivery system. The ciprofloxacin-loaded hydroxyapatite and zinc-doped hydroxyapatite have a good antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus. Hydroxyapatite and zinc-doped hydroxyapatite were prepared and characterized using X-ray diffraction, Transmission electron microscopy and inductively coupled plasma optical emission spectrometry. They were loaded with ciprofloxacin using optimized drug loading parameters. Drug loading, in vitro drug release and antimicrobial activity were analyzed. The influence of zinc on the controlled release of ciprofloxacin was analyzed. The results show that the presence of zinc increases the drug release percentage and that the drug was released in a controlled manner

A study of surface properties of calcium phosphate by means of photoacoustic spectroscopy (FT-IR/PAS), potentiometric titration and electrophoretic measurements

The surface charge at the calcium phosphate/electrolyte interface is created as a result of the various reactions of surface groups and unequal adsorption of ions of an opposite charge from the solution. Photoacoustic spectroscopy (FT-IR/PAS) has been used for identification of different groups of hydroxy- apatite samples. A complementary study of electrical double layer properties was carried out using potentiometric titration and electrophoretic measurements

In vivo application of foam titanium implants with magnesium-doped octacalcium phosphate and hydroxyapatite thin films deposited by PLD method

Results of investigations into integration of foam titanium implants (FTI), with rabbit bone are presented. Three groups of implants were examined: clean titanium implants produced by selective laser melting, FTI coated with a thin film of octacalcium phosphate (OCP), doped with 0.6 weight percent of Mg, deposited by pulsed laser deposition (PLD), and FTI with hydroxyapatite (HA) doped with 0.6 wt.% of Mg. Analysis of histopathology of implants' integration with bone were performed by staining using the triple chromatographic method of Masson - Goldner, pla- nimetric analysis using the Kruskal - Wallis test, an ANOVA test and microtomography. The best results were obtained for implants coated with HA, followed by those coated with OCP. Implants from these two groups showed superior integration with bone than uncoated titanium implants

Functionalized tricalcium phosphate and poly(3-hydroxyoctanoate) derived composite scaffolds as platforms for the controlled release of diclofenac

Novel bone substitutes such as highly porous ceramic scaffolds can serve as platforms for delivering active molecules. A common problem is to control the release of the drug, therefore, it is beneficial to use a drug-functionalized polymer coating. In this study, β-tricalcium phosphate-based porous scaffolds were obtained and coated with diclofenac-functionalized biopolymer – poly(3-hydroxyoctanoate) – P(3HO). To the best of our knowledge, studies using P(3HO) as a component in ceramic-polymer based drug delivery system for bone tissue regeneration have not yet been reported. Presented materials were comprehensively investigated by various techniques such as powder X-ray diffraction, scanning electron microscopy with energy dispersive spectroscopy, hydrostatic weighing and compression tests, pH and ionic conductivity measurements, high-performance liquid chromatography and in vitro cytotoxicity studies. The obtained diclofenac-loaded composite was not only characterised by controlled and sustained drug release, but also possessed improved mechanical properties. Moreover, the precipitation of apatite-like forms on its surface was observed after incubation in simulated body fluid, which indicates its bioactive potential. After 24 hours no cytotoxic effect on MC3T3-E1 mouse preosteoblastic cells was confirmed using indirect cytotoxicity studies. Thus, this promising multifunctional composite scaffold can be a promising candidate as an anti-inflammatory drug-delivery system in bone tissue engineering

Fabrication of Macrochannelled-Hydroxyapatite Bioceramic by a Coextrusion Process

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/65282/1/j.1151-2916.2002.tb00500.x.pd